Du Bois is a village located in Pawnee County, Nebraska. Du Bois has a 2026 population of 123. Du Bois is currently growing at a rate of 0.82% annually and its population has decreased by 0% since the most recent census, which recorded a population of 123 in 2020.
The median age in Du Bois is 53.8 years: 55.3 years for males, and 40.8 years for females. For every 100 females there are 130.2 males.