50 cities
| City | State | 2026 Pop. ↓ | Annual Change | Rank | Type |
|---|---|---|---|---|---|
| Atlanta | Georgia | 530,106 | 0.96% | 1 | City |
| New Orleans | Louisiana | 352,364 | -1.45% | 2 | City |
| Huntsville | Alabama | 237,413 | 1.5% | 3 | City |
| Baton Rouge | Louisiana | 218,223 | -0.61% | 4 | City |
| Augusta | Georgia | 201,590 | -0.04% | 5 | Township |
| Columbus | Georgia | 199,237 | -0.65% | 6 | City |
| Mobile | Alabama | 198,806 | -0.64% | 7 | City |
| Birmingham | Alabama | 194,400 | -0.5% | 8 | City |
| Montgomery | Alabama | 193,703 | -0.54% | 9 | City |
| Shreveport | Louisiana | 171,469 | -1.47% | 10 | City |
| Charleston | South Carolina | 160,965 | 1.04% | 11 | City |
| Macon-Bibb County | Georgia | 157,065 | 0% | 12 | Township |
| Savannah | Georgia | 149,465 | 0.22% | 13 | City |
| Columbia | South Carolina | 149,078 | 1.46% | 14 | City |
| Metairie | Louisiana | 139,114 | 0.03% | 15 | CDP |
| Jackson | Mississippi | 135,671 | -2.09% | 16 | City |
| North Charleston | South Carolina | 131,201 | 2.02% | 17 | City |
| Athens | Georgia | 129,417 | 0.28% | 18 | Township |
| Lafayette | Louisiana | 122,695 | 0.17% | 19 | City |
| Tuscaloosa | Alabama | 118,643 | 1.87% | 20 | City |
| South Fulton | Georgia | 113,884 | 0.83% | 21 | City |
| Sandy Springs | Georgia | 104,151 | -0.65% | 22 | City |
| Mount Pleasant | South Carolina | 97,780 | 1.13% | 23 | Town |
| Hoover | Alabama | 93,174 | 0.09% | 24 | City |
| Roswell | Georgia | 91,871 | -0.19% | 25 | City |
| Warner Robins | Georgia | 89,009 | 1.6% | 26 | City |
| Auburn | Alabama | 87,102 | 1.96% | 27 | City |
| Johns Creek | Georgia | 80,458 | -0.44% | 28 | City |
| Lake Charles | Louisiana | 79,293 | -1.16% | 29 | City |
| Rock Hill | South Carolina | 76,370 | 0.38% | 30 | City |
| Greenville | South Carolina | 75,842 | 0.98% | 31 | City |
| Gulfport | Mississippi | 75,491 | 0.58% | 32 | City |
| Dothan | Alabama | 71,922 | 0.19% | 33 | City |
| Alpharetta | Georgia | 67,929 | 0.48% | 34 | City |
| Madison | Alabama | 67,388 | 2.56% | 35 | City |
| Albany | Georgia | 65,506 | -0.91% | 36 | City |
| Marietta | Georgia | 64,165 | 0.82% | 37 | City |
| Kenner | Louisiana | 63,830 | -0.64% | 38 | City |
| Bossier City | Louisiana | 63,471 | 0.2% | 39 | City |
| Stonecrest | Georgia | 61,888 | 0.71% | 40 | City |
| Brookhaven | Georgia | 60,666 | 1.08% | 41 | City |
| Southaven | Mississippi | 58,816 | 1.14% | 42 | City |
| Decatur | Alabama | 58,394 | 0.36% | 43 | City |
| Smyrna | Georgia | 57,682 | 0.44% | 44 | City |
| Valdosta | Georgia | 55,788 | 0.11% | 45 | City |
| Summerville | South Carolina | 53,459 | 0.79% | 46 | Town |
| Goose Creek | South Carolina | 52,398 | 1.99% | 47 | City |
| Dunwoody | Georgia | 51,894 | 0.1% | 48 | City |
| Greer | South Carolina | 51,559 | 5.36% | 49 | City |
| Gainesville | Georgia | 50,467 | 2.81% | 50 | City |
For this list, the Deep South Region is defined as the 5 states of Alabama, Georgia, Louisiana, Mississippi and South Carolina.